Dorota Skowyra, Ph.D.

Associate Professor
Biochemistry and Molecular Biology


Research

Studies on the role, function, and regulation of the ubiquitin-proteasome system in health and disease.

Research Interests

We are broadly interested in the role, function and regulation of the ubiquitin-proteasome system. Our former projects focused on the mechanism by which proteins are recruited for degradation by the 26S proteasome in yeast. We have recently initiated three new research directions that focus on the role of proteasomal proteolysis in 1) autoimmune diseases (type 1 diabetes); 2) protein misfolding diseases (liver disease associated with alpha 1 antitrypsin deficiency); and early antiviral responses (ectromelia virus/primary mouse macrophages). Biochemical and cellular approaches are our primary research tools.

Publications

Recent

Erratum to: The loop-less ™Cdc34 E2 mutant defective polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2
Lass A, Cocklin R, Scaglione KM, Skowyra M, Korolev S, Goebl M and Skowyra D
Pubmed | Cell Div

PiZ mouse liver accumulates polyubiquitin conjugates that associate with catalytically active 26S proteasomes
Haddock CJ, Blomenkamp K, Gautam M, James J, Mielcarska J, Gogol E, Teckman J and Skowyra D
Pubmed | PLoS ONE

Immunoproteasome Activation During Early Antiviral Response in Mouse Pancreatic β-cells: New Insights into Auto-antigen Generation in Type I Diabetes?
Freudenburg W, Gautam M, Chakraborty P, James J, Richards J, Salvatori AS, Baldwin A, Schriewer J, Buller RM, Corbett JA and Skowyra D
Pubmed | J Clin Cell Immunol

Reduction in ATP levels triggers immunoproteasome activation by the 11S (PA28) regulator during early antiviral response mediated by IFNβ in mouse pancreatic β-cells
Freudenburg W, Gautam M, Chakraborty P, James J, Richards J, Salvatori AS, Baldwin A, Schriewer J, Buller RM, Corbett JA and Skowyra D
Pubmed | PLoS ONE

The loop-less tmCdc34 E2 mutant defective in polyubiquitination in vitro and in vivo supports yeast growth in a manner dependent on Ubp14 and Cka2
Lass A, Cocklin R, Scaglione KM, Skowyra M, Korolev S, Goebl M and Skowyra D
Pubmed | Cell Div