SLUCare's DNA Diagnostic Laboratory offers a variety of tests that utilize molecular biology techniques for genetic, microbiological and oncological analysis. These techniques allow identification of gene rearrangements in neoplastic processes, detection of infectious agents and the direct detection of specific inherited mutations.
Southern blot and PCR offer highly specific and sensitive methods of detection in the evaluation of neoplasms. DNA analysis is available for biopsied samples of lymphoid, peripheral blood, bone marrow and other tumors in order to detect characteristic gene rearrangements and the activation of specific oncogenes.
For clinical microbiology, DNA diagnosis by PCR offers a highly specific and sensitive identification of pathogenic microorganisms, which may be especially difficult to culture in the clinical laboratory. Direct genetic diagnosis requires a specimen from the individual for detection or corroboration of a suspected inherited disorder.
- Direct Genetic Diagnosis: The most common mutations found in hereditary hemochromatosis, the Coagulopathy-Associated Activated Protein C Resistance Mutation of Factor V, Coagulopathy associated mutation of the Prothrombin gene, and the temperature-sensitive mutation of methylenetetrahydrofolate reductase. IL28B polymorphisms affecting response to anti-hepatitis C therapy.
- DNA Diagnosis in Microbiology/Virology: Herpes simplex virus, including the typing of the pathogen in cerebrospinal fluid specimens. Detection, quantitation and genotyping of hepatitis C in peripheral blood. Detection of high-risk HPV, CT/NG in ThinPrep cervical specimens, urine or swabs. Detection of EBV DNA in peripheral blood. Detection/quantitation of BK and CMV virus in serum/urine and plasma.
- DNA Diagnosis in Cancer: T-cell monoclonality, B-cell monoclonality, Philadelphia chromosome (bcr/abl) translocation and bcl-2 rearrangements in lymphoma. JAK2 mutations associated with chronic myeloproliferative disorders. EGFR Exon 21 point mutations and deletions of Exon 19 in lung cancer. K-ras, BRAF mutations in cancer. Microsatellite Instability in colon cancer and inherited cancer syndromes and FLT3 and NPMI mutations for AML. Amplification of Her2/Neu by in situ hybridization in breast and gastroesophageal cancer.
The DNA Diagnostic Laboratory maintains an expanding list of tests. The staff also assists in sending samples to and interpreting results from other certifiable laboratories for tests not offered by our laboratory.
Hours of Operation
The DNA Diagnostic Laboratory is open 8 a.m. to 4:30 p.m. Monday through Friday.
- Director Telephone: (314) 577-8482
- Office Telephone: (314) 577-8344
- Fax: (314) 268-5598
- Mail: DNA Diagnostic Laboratory, Saint Louis University Hospital West Pavilion, 3635 Vista Ave.,
St. Louis, MO 63110-2539
DNA Available Tests
Prior arrangements with the laboratory may be necessary for special procedures or scheduling. For such arrangements, please contact the DNA Diagnostic Laboratory at (314) 577-8344 or, director of the DNA Laboratory, at (314) 577-8482.
Written reports with interpretation are generally available within two working weeks after the beginning of testing. Final reports are available online and are sent to the physician and added to the patient's chart; in addition the results of the assay can be accessed through Meditech in the Anatomic Pathology directory. HCV, K-RAS and EGFR results are located in the laboratory module of Meditech. Partial results and plans for further testing, if needed, are discussed by the director with the referring physician.