The high-speed cell sorter in the flow cytometry research core facility has undergone a major upgrade.
The upgrade of the current BD Biosciences FACSAria I sorter to a FACSAria IIIu ("u" for upgrade) includes a completely new fluidics system, new computer hardware and software and the addition of a fourth laser (561 nm).
It will not only extend the life of the FACS Aria, but also improve its operation and enhance its flexibility and capability, providing SLU researchers with one of the most versatile, state-of-the-art sorters available today.
The new fluidics system will provide our users a much more stable and reliable instrument. The new computer system and software upgrades will allow better monitoring of instrument performance.
Finally, the addition of a 561-nm laser will expand the available choices of fluorescent markers that researchers may use and improve the sensitivity of detection of other commonly used fluorochromes.
With the new laser, users will be able to sort cells using various red fluorescent proteins and "fruit" fluorescent proteins, such as mCherry, and fluorochromes like Texas-Redïƒ' and Alexa Fluorïƒ' 594.
The incorporation of this laser also provides the capability to physically separate the excitation and emission of two commonly used fluorochromes, FITC and PE, resulting in the elimination of spillover between emission spectra of these two fluorochromes. The FACSAria IIIu will now have 488-nm, 561-nm, 633-nm, and 407-nm lasers available for excitation and have two additional slots open for possible future expansion.
The flow cytometry core is located on the 7th floor of the Doisy Research Center and serves researchers from many different departments in the School of Medicine.
To learn more about the new sorter and any of our other instruments or to discuss ways to use flow cytometry to advance your research, please contact core manager, Joy Eslick, at 977-8877, or core director, Mike Green, PhD., at 977-8897.