Anping Chen, Ph.D., associate professor of pathology, will present "The Implications and Mechanisms of Hyperinsulinemia and Curcumin in the Activation of Hepatic Stellate Cells and Hepatic Fibrogenesis" at noon Monday, March 14, during Topics in Pathology in Pitlyk Auditorium C, Learning Resources Center.
|Anping Chen, Ph.D.|
|Insulin stimulates the activation of quiescent hepatic stellate cells (HSCs) in vitro, which is attenuated by curcumin. After culturing for 24 hr in serum-rich media (20% FBS) for cell attachment, freshly-isolated HSCs were cultured in DMEM with 10% FBS, or 2% FBS plus insulin (100 nM) with or without curcumin at indicated concentrations for additional 1, 3, or 5 days. After fixation, the cells were stained with Oil Red O for detecting intracellular lipid droplets, which were colored dark red by Oil Red O. Representative views were presented. (Lin J, Zheng S, and Chen C, Lab Invest 89:1397-1409, 2009)|